CmoNAC1 in pumpkin rootstocks improves salt tolerance of grafted cucumbers by binding to the promoters of <i>CmoRBOHD1</i>/<i>CmoNCED6</i> and <i>CmoAKT1;2</i>/<i>CmoHKT1;1</i> to regulate H2O2/ABA signaling and K+/Na+ homeostasis

Abstract The NAC transcription factor is a type of plant-specific that can regulate plant salt tolerance, but the underlying mechanism unclear in grafted vegetables. H2O2 and ABA pumpkin rootstocks be transported to cucumber scion leaves, promoting stomatal closure improve tolerance cucumbers. Despite these observations, regulatory unknown. Here, our research revealed CmoNAC1 key regulates signaling roots under stress. function was analyzed using root transformation RNA-seq, we found promoted production via CmoRBOHD1 CmoNCED6, respectively, regulated K+/Na+ homeostasis CmoAKT1;2, CmoHKT1;1 CmoSOS1 Root knockout resulted significant decrease (52.9% 32.1%)/ABA (21.8% 42.7%) content ratio (81.5% 56.3%) leaf cucumber, while overexpression showed opposite effect. experiment CmoNCED6 could cucumbers by regulating Finally, bound promoters CmoRBOHD1, CmoAKT1;2 yeast one-hybrid, luciferase, electrophoretic mobility shift assays. In conclusion, not only binds signals roots, also increase ratio, thus improving